食料バイオテクノロジー研究室

立命館大学 生命科学部 生物工学科

原著論文(すべて査読有り)

  1. Kishi-Kaboshi M., Muto H., Takeda A., Murata T., Hasebe M. and Watanabe Y. (2014) Characterization of tobacco germin-like protein 1 in leaf intercellular space. Plant Physiology and Biochemistry 85, 1–8.
  2. Tsuzuki M., Takeda A. and Watanabe Y. (2014) Recovery of dicer-like 1-late flowering phenotype by miR172 expressed by the non-canonical DCL4-dependent biogenesis pathway. RNA 20, 1320–1327.
  3. Carbonell A., Takeda A., Fahlgren N., Johnson S. C., Cuperus J. T. and Carrington J. C. (2014) New generation of artificial microRNA and synthetic trans-acting small interfering RNA vectors for efficient gene silencing in Arabidopsis. Plant Physiology 16, 15–29.
  4. Kumakura N., Otsuki H., Tsuzuki M., Takeda A. and Watanabe Y. (2013) Arabidopsis AtRRP44A is the functional homolog of Rrp44/Dis3, an exosome component, is essential for viability and is required for RNA processing and degradation. PLOS One 8, e79219.
  5. Hayashi M., Nanba C., Saito M., Kondo M., Takeda A., Watanabe Y. and Nishimura M. (2012) Loss of XRN4 Function can trigger cosuppression in a sequence-dependent manner. Plant and Cell Physiology 53, 1310–1321.
  6. Motomura K., Le Q., Kumakura N., Fukaya T., Takeda A., and Watanabe Y. (2012) The role of decapping proteins in the miRNA accumulation in Arabidopsis thaliana. RNA Biology 9, 644–652.
  7. Mine A., Hyodo K., Takeda A., Kaido M., Mise K. and Okuno T. (2010) Interactions between p27 and p88 replicase proteins of Red clover necrotic mosaic virus play an essential role in viral RNA replication and suppression of RNA silencing via the 480-kDa viral replicase complex assembly. Virology 407, 213–224.
  8. Cuperus J. T., Carbonell A., Fahlgren N., Garcia-Ruiz H., Burke R. T., Takeda A., Sullivan C. M., Gilbert S. D., Montgomery T. A. and Carrington J. C. (2010) Unique functionality of 22-nt miRNAs in triggering RDR6-dependent siRNA biogenesis from target transcripts in Arabidopsis. Nature Structural and Molecular Biology 17, 997–1004.
  9. Mine A., Takeda A., Taniguchi T., Taniguchi H., Kaido M., Mise K. and Okuno T. (2010) Identification and characterization of the 480 kilodalton template-specific RNA-dependent RNA polymerase complex of Red clover necrotic mosaic virus. Journal of Virology 84, 6070–6081.
  10. Dunoyer P., Schott G., Himber C., Meyer D., Takeda A., Carrington J. C. and Voinnet O. (2010) Small RNA duplexes function as mobile silencing signals between plant cells. Science 328, 912–916.
  11. Garcia-Ruiz H., Takeda A., Chapman E. J., Sullivan C. M., Fahlgren N., Brempelis K. J. and Carrington J. C. (2010) Arabidopsis RNA-dependent RNA polymerases and Dicer-like proteins in antiviral defense and siRNA biogenesis during Turnip Mosaic Virus infection. Plant Cell 22, 481–496.
  12. Kumakura N., Takeda A., Fujioka Y., Motose H., Takano R. and Watanabe Y. (2009) SGS3 and RDR6 interact and colocalize in cytoplasmic SGS3/RDR6-bodies. FEBS Letters 583, 1261–1266.
  13. Okamoto K., Nagano H., Iwakawa H., Mizumoto H., Takeda A., Kaido M., Mise K. and Okuno T. (2008) cis-preferencial requirement of a -1 frameshift product p88 for the replication of Red clover necrotic mosaic virus RNA1. Virology 375, 205–212.
  14. Takeda A., Iwasaki S., Watanabe T., Utsumi M. and Watanabe Y. (2008) The mechanism selecting the guide strand from small RNA duplexes is different among Argonaute proteins. Plant and Cell Physiology 49, 493–500.
  15. Akamatsu N., Takeda A., Kishimoto M., Kaido M., Okuno T. and Mise K. (2007) Phosphorylation and interaction of the movement and coat proteins of brome mosaic virus in infected barley protoplasts. Archives of Virology 152, 2087–2093.
  16. Kaido M., Inoue Y., Takeda Y., Sugiyama K., Takeda A., Mori M., Tamai A., Meshi T., Okuno T. and Mise K. (2007) Downregulation of the NbNACa1 gene encoding a movement protein-interacting protein reduces cell-to-cell movement of Brome mosaic virus in Nicotiana benthamiana. Molecular Plant-Microbe Interactions 20, 671–681.
  17. Kurihara Y., Inaba N., Kutsuna N., Takeda A., Tagami Y. and Watanabe Y. (2007) Binding of tobamovirus replication protein with small RNA duplexes. Journal of General Virology 88, 2347–2352.
  18. Iwasaki S., Takeda A., Motose H. and Watanabe Y. (2007) Characterization of Arabidopsis decapping proteins AtDCP1 and AtDCP2, which are essential for post-embryonic development. FEBS Letters 581, 2455–2459.
  19. Watanabe T., Takeda A., Tsukiyama T., Mise K., Okuno T., Sasaki H., Minami N. and Imai H. (2006) Identification and characterization of two novel classes of small RNAs in the mouse germline: retrotransposon-derived siRNAs in oocytes and germline small RNAs in testes. Genes & Development 20, 1732–1743.
  20. Iwamoto T., Mise K., Takeda A., Okinaka Y., Mori K. -I., Arimoto M., Okuno T. and Nakai T. (2005) Characterization of Striped jack nervous necrosis virus subgenomic RNA3 and biological activities of its encoded protein B2. Journal of General Virology 86, 2807–2816.
  21. Takeda A.*, Tsukuda M.*, Mizumoto H., Okamoto K., Kaido M., Mise K. and Okuno T. (2005) A plant RNA virus suppresses RNA silencing through viral RNA replication. The EMBO Journal 24, 3147–3157.
  22. Takeda A.*, Nakamura W.*, Sasaki N., Kaido M., Mise K. and Okuno T. (2005) Natural isolates of Brome mosaic virus with the ability to move from cell to cell independently of coat protein. Journal of General Virology 86, 1201–1211.
  23. Watanabe T., Takeda A., Mise K., Okuno T., Suzuki T., Minami N. and Imai H. (2005) Stage-specific expression of microRNAs during Xenopus development. FEBS Letters 579, 318–324.
  24. Takeda A., Kaido M., Mise K. and Okuno T. (2004) The C terminus of the movement protein of Brome mosaic virus controls the requirement for coat protein in cell-to-cell movement and plays a role in long-distance movement. Journal of General Virology 85, 1751–1761.
  25. Takeda A., Sugiyama K., Nagano H., Mori M., Kaido M., Mise K., Tsuda S. and Okuno T. (2002) Identification of a novel RNA silencing suppressor, NSs protein of Tomato spotted wilt virus. FEBS Letters 532, 75–79.

総説・解説

  1. Takeda A. (2014) Studies on molecular mechanisms of RNA silencing-mediated anti-virus defense in plants and RNA silencing suppression by plant viruses. Journal of General Plant Pathology in press.
  2. 竹田 篤史 (2014) RNAサイレンシングによるウイルス抵抗性機構及びウイルスによるRNAサイレンシング抑制機構に関する研究. 日本植物病理学会報 80, 22.
  3. 竹田 篤史 (2011) 低分子二本鎖RNAは植物細胞間の移行性サイレンシングシグナルとして機能する. Science-Japanese Scientists in Science 2010-Science誌に載った日本人研究者 31.
  4. 竹田 篤史 (2010) 植物のmiRNAとsiRNA経路. 拡大・進展を続けるRNA研究の最先端(実験医学増刊)28, 1573–1578.
  5. 奥野 哲郎、竹田 篤史 (2007) ウイルスとRNAサイレンシング. 植物における環境と生物ストレスに対する応答(蛋白質核酸酵素増刊) 52, 692–697.
  6. 渡部 聡朗、竹田 篤史 (2007) マウス生殖細胞で発現するpiRNAとsiRNA. 実験医学 25, 800–805.
  7. 竹田 篤史、渡邊 雄一郎 (2006) 植物のsmall RNAに関する話題. RNAと生命(蛋白質核酸酵素増刊)51, 2463–2470.
  8. Takeda A., Mise K. and Okuno T. (2005) RNA silencing suppressors encoded by viruses of the family Tombusviridae. Plant Biotechnology 22, 447–454.
  9. 竹田 篤史、三瀬 和之、奥野 哲郎 (2005) 植物ウイルスとRNAi. 化学と生物 43, 468–475.

受賞歴

  • 2014年度、日本植物病理学会学術奨励賞
  • 2010年度、日本植物生理学会PCP論文賞

学会発表(2014以降)

2014
   1. miR172強制発現によるシロイヌナズナDicer-like 1変異体の部分相補
     都筑正行、竹田篤史、渡邊雄一郎
     日本植物学会第78回大会、P-174
   2. 植物microRNAの特異性に関する研究
     唐戸俊介、都筑正行、白谷公孝、渡邊雄一郎、竹田篤史
     日本植物学会第78回大会、P-198
   3. RNAサイレンシングによるウイルス抵抗性機構及びウイルスによるRNAサイレンシング抑制機構
     に関する研究
     竹田篤史
     第99回日本植物病理学会年会、奨励賞受賞講演