細胞工学研究室

立命館大学 薬学部 細胞工学研究室のホームページ

細胞工学研究室について

々ヒトの体は、約200種類からなる約37兆個の細胞により構成され、これらが1つのシステム(個体)として統合されています。これらの基となった細胞、ゲノム情報は1つであり、発生の過程でこのような多種類、多数の細胞が生み出されます。ES、iPS細胞は自己複製能と多分化能を保持したまま、in vitroで培養できる細胞のため、幹細胞細胞分化研究における有力なツールす。細胞工学研究室では、魚類からマウス、サル、ヒトに至る幅広い生物種の幹細胞(生殖幹細胞、ES、iPS細胞)を用いて、細胞分化におけるシグナル環境に関する研究、および幹細胞生物学を環境科学、創薬、生物資源保存に応用した研究を展開しています。

Projects

Transcriptome analysis revealed bisphenol A and nonylphenol affect reproduction

We investigated the effect of Bisphenol A (BPA) and Nonylphenol (NP) on cell differentiation, comparing transcriptome of EDC treated ES cells to that of normal germ cell development deposited in GEO data base. GO-based gene set enrichment analysis revealed that EDCs functionally affect wide range of reproduction by the modification of gene expression of germ cell specific genes especially related to meiosis.


Reproductive Toxicology 2019DOI: 10.1016/j.reprotox.2019. 06.006

In vitro differentiation of fertile sperm from cryopreserved spermatogonia of the endangered endemic cyprinid honmoroko (Gnathopogon caerulescens)

We succeeded the in vitro differentiation of fertile sperm from cryopreserved spermatogonia isolated from juveniles of the endangered small cyprinid honmoroko (Gnathopogon caerulescens), which is endemic to Lake Biwa in Japan


Scientific Reports 2017DOI:10.1038/srep42852

DNA methylation dynamics in mouse preimplantation embryos revealed by mass spectrometry

We here report direct quantification of genomic 5-methyl-2′-deoxycytidine (5 mC) and 5-hydroxymethyl-2′-deoxycytidine (5 hmC) and their dynamics of mouse embryos during preimplantation development using small scale liquid chromatographic tandem mass spectrometry (SMM)


Scientific Reports 2016DOI: 10.1038/srep19134

Response to fish specific reproductive hormones and endocrine disrupting chemicals of a Sertoli cell line expressing endogenous receptors from an endemic cyprinid Gnathopogon caerulescens

We established an epithelium-like cell line RME1 from the blastula-stage embryos of the critically endangered cyprinid Honmoroko (Gnathopogon caerulescens), which is endemic to ancient Lake Biwa in Japan.


Gen Comp Endocrinol 2013DOI: 10.1016/j.ygcen.2013.06. 002

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